data analysis software for perpendicular line scanning fluorescence correlation spectroscopy.

When a membrane is scanned perpendicularly to its surface, the fluorescence signal originating from the membrane itself must be separated from the signal of the surrounding medium for an FCS analysis. PyScanFCS interactively extracts the fluctuating fluorescence signal from such measurements and applies a multiple-tau algorithm. The obtained correlation curves can be evaluated using PyCorrFit.

Supported Operating Systems

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How to cite

PyScanFCS is described in

Müller, P., Schwille, P., and Weidemann, T.
Fluorescence Spectroscopy and Microscopy, chapter: Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane. Methods in Molecular Biology, Humana Press, Springer, New York. 2014, 635-651. DOI:10.1007/978-1-62703-649-8_29
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PyScanFCS and PyCorrFit were both developed by Paul Müller.

Affiliation:
I currently work in the group of Prof. Jochen Guck in the Biotechnology Center of the TU Dresden.

PyScanFCS was partly developed in the group of Petra Schwille.