When a membrane is scanned perpendicularly to its surface, the fluorescence signal originating from the membrane itself must be separated from the signal of the surrounding medium for an FCS analysis. PyScanFCS interactively extracts the fluctuating fluorescence signal from such measurements and applies a multiple-tau algorithm. The obtained correlation curves can be evaluated using PyCorrFit.
PyScanFCS is described in
|Müller, P., Schwille, P., and Weidemann, T.|
|Fluorescence Spectroscopy and Microscopy, chapter: Scanning Fluorescence Correlation Spectroscopy (SFCS) with a Scan Path Perpendicular to the Membrane Plane. Methods in Molecular Biology, Humana Press, Springer, New York. 2014, 635-651. DOI:10.1007/978-1-62703-649-8_29|
|Download Author’s Manuscript|
PyScanFCS was partly developed in the group of Petra Schwille.